Effect of sleep restriction on the human transcriptome during extended wakefulness



In a cross balanced design human subjects were given one week of sufficient sleep and insufficient sleep (6 h per day). Following each of these conditions they were then kept awake for a day, a night and the subsequent day. During each of these periods of extended wakefulness 10 blood samples were taken, RNA was extracted from leukocytes, labelled and hybridised to human whole-genome microarrays

Overall Design

A total of 427 samples comprising 26 human subjects, for which 20 samples across multiple time-points/sleep condition were collected. We have focussed on two genotypes related to the variable number tandem repeat (VNTR) polymorphism of the circadian gene PERIOD3 (PER3): a 4-repeat VNTR allele of PER3 (PER3(4/4)) and a 5-repeat VNTR allele of PER3 (PER3(5/5)).


Data and Resources

Raw Files [427]

Additional Info

Field Value
Source https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39445
Type of Data

Expression profiling by array



GSE Submission Date 17/07/2012
GSE Authors Carla,,Moller-Levet; Simon Archer; Giselda Bucca; Emma Laing; Ana Slak; Renata Kabiljo; June Lo; Nayantara Santhi; Malcolm von Schantz; Colin,P,Smith; Derk-Jan Dijk
Pubmed ID 23440187
Dataset Last Updated December 2, 2020, 14:59 (UTC)
Dataset Created November 29, 2019, 13:01 (UTC)